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Ridom GmbH staphtype software program
Staphtype Software Program, supplied by Ridom GmbH, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/staphtype+software+program/staphtype+software/pm34635425-28-55-61
Average 90 stars, based on 1 article reviews
staphtype software program - by Bioz Stars, 2026-09
90/100 stars

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Software:

Article Title: Molecular Typing and Virulence Characteristic of Methicillin-Resistant Staphylococcus Aureus Isolates from Pediatric Patients in Bucaramanga, Colombia
Article Snippet: .. The sequences were analyzed with the Ridom StaphType software program (version 1.4; Ridom, GmbH, Wurzburg, Germany [ http://spa.ridom.de/index.shtml ]) to obtain the spa type for each strain. ..

Article Title: Molecular epidemiology and phenotypes of invasive methicillin-resistant vancomycin-intermediate Staphylococcus aureus in Taiwan.
Article Snippet: .. Polymorphism of the X region encoding protein A (spa typing) The X region of the spa gene contains variable numbers of 21- to 27-base pair (bp) repeats, among which 24-bp repeats are most common.18 The X region of each MRSA was amplified by PCR.19 and the amplified products were then sequenced and analyzed by the Ridom StaphType software program (version 1.4; Ridom, GmbH, Wurzburg, Germany [http://spa.ridom.de/index.shtml]), which automatically determined the repeat profile and the spa type of each isolate.20 Polymorphism of the mec-associated hypervariable region Amplification of the hypervariable region of SCCmec, which contains variable numbers of 40-bp direct repeat units (dru), was performed by PCR with orf145 and IS431mec primers.21 The dru copy number in each MRSA was determined by the size of the amplified fragment according to the following equation: dru copy no. Z (size of PCR product - 171) O 40.21 Accessory gene regulator (agr) typing The agr gene of each S. aureus isolate was amplified with specialized primers (Pan, agr1, agr2, agr3, and agr4).22 These primers were designed to amplify a 441-bp DNA fragment from agr group I, a 575-bp fragment from agr group II, a 323-bp fragment from agr group III, and a 659-bp fragment from agr group IV strains. ..

Article Title: Distribution of Antiseptic Resistance GenesqacA, qacB, andsmrin Methicillin-Resistant Staphylococcus aureus Isolated in Toronto, Canada, from 2005 to 2009
Article Snippet: .. The Ridom StaphType software program was used for analysis and assignment of a spa type to each isolate. ..

Article Title: Comparing Whole-Genome Sequencing with Sanger Sequencing for spa Typing of Methicillin-Resistant Staphylococcus aureus
Article Snippet: .. The introduction of the Ridom StaphType software program in 2003 ( 3 ) has made spa typing an excellent tool to compare MRSA types across regions and countries, since it has proven to be a typing scheme with high interlaboratory reproducibility ( 4 ). spa typing is the sequencing of the 24-bp (21 to 30 bp) repeat region of the Staphylococcus protein A gene ( spa ). ..

Article Title: A Longitudinal Case Study on Dissemination of ST398 Methicillin-Resistant Staphylococcus aureus Within a Dairy Cow Herd.
Article Snippet: The study was conducted to describe the dynamics of ST398 methicillin-resistant Staphylococcus aureus (MRSA) on a dairy herd in northeastern Italy.. MRSA was first identified in this herd of 120 cows in 2016, after which the herd was sampled once every 3 months for 1 year (April 2016–May 2017).. Samples collected included nasal swabs and milk samples from cows and nasal swabs from farmworkers.

Article Title: Molecular Typing and Phenotype Characterization of Methicillin-Resistant Staphylococcus aureus Isolates from Blood in Taiwan
Article Snippet: .. The amplified products were sequenced and analyzed by the Ridom StaphType software program (version 1.4; Ridom, GmbH, Wurzburg, Germany [ http://spa.ridom.de/index.shtml ]), which automatically determined the repeat profile and the spa type of each isolate . ..

other:

Article Title: Short communication: Methicillin-resistant Staphylococcus aureus in bulk tank milk of dairy cows and effect of swine population density.
Article Snippet: To obtain the spa type, the sequences were analyzed with the Ridom StaphType soft- ware program (version 1.4; Ridom, GmbH, Wurzburg, Germany, http://spa.ridom.de/index.shtml).

Article Title: Molecular Characterization and Panton-Valentine Leucocidin Typing of Community-Acquired Methicillin-Sensitive Staphylococcus aureus Clinical Isolates
Article Snippet: Genotyping using the spa technique revealed 11 types clustered into 2 spa clonal clusters (CCs) (CC005 and CC345/657) and 5 singletons based on the BURP algorithm (StaphType software program; Ridom GmbH, Wurzburg, Germany).

Amplification:

Article Title: Molecular epidemiology and phenotypes of invasive methicillin-resistant vancomycin-intermediate Staphylococcus aureus in Taiwan.
Article Snippet: .. Polymorphism of the X region encoding protein A (spa typing) The X region of the spa gene contains variable numbers of 21- to 27-base pair (bp) repeats, among which 24-bp repeats are most common.18 The X region of each MRSA was amplified by PCR.19 and the amplified products were then sequenced and analyzed by the Ridom StaphType software program (version 1.4; Ridom, GmbH, Wurzburg, Germany [http://spa.ridom.de/index.shtml]), which automatically determined the repeat profile and the spa type of each isolate.20 Polymorphism of the mec-associated hypervariable region Amplification of the hypervariable region of SCCmec, which contains variable numbers of 40-bp direct repeat units (dru), was performed by PCR with orf145 and IS431mec primers.21 The dru copy number in each MRSA was determined by the size of the amplified fragment according to the following equation: dru copy no. Z (size of PCR product - 171) O 40.21 Accessory gene regulator (agr) typing The agr gene of each S. aureus isolate was amplified with specialized primers (Pan, agr1, agr2, agr3, and agr4).22 These primers were designed to amplify a 441-bp DNA fragment from agr group I, a 575-bp fragment from agr group II, a 323-bp fragment from agr group III, and a 659-bp fragment from agr group IV strains. ..

Article Title: Molecular Typing and Phenotype Characterization of Methicillin-Resistant Staphylococcus aureus Isolates from Blood in Taiwan
Article Snippet: .. The amplified products were sequenced and analyzed by the Ridom StaphType software program (version 1.4; Ridom, GmbH, Wurzburg, Germany [ http://spa.ridom.de/index.shtml ]), which automatically determined the repeat profile and the spa type of each isolate . ..

Polymerase Chain Reaction:

Article Title: Molecular epidemiology and phenotypes of invasive methicillin-resistant vancomycin-intermediate Staphylococcus aureus in Taiwan.
Article Snippet: .. Polymorphism of the X region encoding protein A (spa typing) The X region of the spa gene contains variable numbers of 21- to 27-base pair (bp) repeats, among which 24-bp repeats are most common.18 The X region of each MRSA was amplified by PCR.19 and the amplified products were then sequenced and analyzed by the Ridom StaphType software program (version 1.4; Ridom, GmbH, Wurzburg, Germany [http://spa.ridom.de/index.shtml]), which automatically determined the repeat profile and the spa type of each isolate.20 Polymorphism of the mec-associated hypervariable region Amplification of the hypervariable region of SCCmec, which contains variable numbers of 40-bp direct repeat units (dru), was performed by PCR with orf145 and IS431mec primers.21 The dru copy number in each MRSA was determined by the size of the amplified fragment according to the following equation: dru copy no. Z (size of PCR product - 171) O 40.21 Accessory gene regulator (agr) typing The agr gene of each S. aureus isolate was amplified with specialized primers (Pan, agr1, agr2, agr3, and agr4).22 These primers were designed to amplify a 441-bp DNA fragment from agr group I, a 575-bp fragment from agr group II, a 323-bp fragment from agr group III, and a 659-bp fragment from agr group IV strains. ..

Sequencing:

Article Title: Comparing Whole-Genome Sequencing with Sanger Sequencing for spa Typing of Methicillin-Resistant Staphylococcus aureus
Article Snippet: .. The introduction of the Ridom StaphType software program in 2003 ( 3 ) has made spa typing an excellent tool to compare MRSA types across regions and countries, since it has proven to be a typing scheme with high interlaboratory reproducibility ( 4 ). spa typing is the sequencing of the 24-bp (21 to 30 bp) repeat region of the Staphylococcus protein A gene ( spa ). ..



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